The best Side of attune flow cytometer
The best Side of attune flow cytometer
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Facts-enriched characterization of cell populations. Morphological data from photos can insert for the richness of flow cytometry details. As an example, the determine demonstrates an usually typical apoptosis assay employing Annexin V and PI, including mobile imaging to characterize cells in Every population to reveal morphologically distinctive functions. These insights couldn't are already attained from multiplex staining on your own.
As samples are obtained over the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and suppliers photos of detected activities at a rate as many as six,000 photographs for every 2nd, according to the flow amount and graphic size. For greater adaptability, Attune Cytometric Software program helps you to change the image seize frequency as necessary and select a specific gate for image capture, if expected.
(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The quantity of singlets available for Investigation will increase once the person trains and employs a far more precise design.
Learn new insights into your experimental facts by visualizing populations making use of merged fluorescence and morphology data. Our application interface wizards empower downsampling, concatenation (merged files) and dimensionality equipment which are designed into your software program for an intuitive, streamlined knowledge Examination knowledge.
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Additionally, it truly is illuminating to determine what number of doublets slip as a result of regular singlet gates, and fantastic to confirm the position of unexpected double-favourable activities in my Assessment."
Pair your Attune flow cytometer with an autosampler for enhanced workflow performance, and add robotic integration for maximum throughput.
Our researchers will design and style panels for you as many times you would like—cost-free, no product invest in wanted.
Early signs of this contamination experienced originally been dismissed as particles. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological attributes to discover attention-grabbing subpopulations that could not be evident from flow cytometry knowledge by yourself.
Most likely most of all, these occasions incorporate cells of Plainly diverse phenotypes which may lead to incorrect conclusions relating to double positive situations (particularly in unusual populations).
Audio waves help exact mobile alignment, leading to Remarkable data good quality and ~10X more quickly sample flow rates.
We Earlier demonstrated the power of imaging Auto-T/Ramos mobile interactions. Let’s examine just the population of greatest fascination, the double optimistic occasions, to learn more. We can easily now use prolonged morphological parameters (listed here, circularity and skewness of intensity) to further more analyze the features of such populations and refine gating on these gatherings, expanding knowledge robustness.
Designed-in dimensionality reduction instruments increased resolution into populations. click here Fluorescently gated populations back again-gated on to UMAP plot to substantiate envisioned population resolution in lysed whole human blood. Consumer can watch functions from Just about every cluster for Visible confirmation of mobile phenotypes.
Adaptable payment approach from Agilent Economical Remedies enable you to have the important instrumentation you'll need for instant challenge, or to expand your lab.
Gating on these prolonged parameters allows you to swiftly and properly discover populations of interest and make sure gating tactic with little if any handbook evaluation. The Attune software program provides use of state-of-the-art equipment Mastering Evaluation resources inside an expertly intended person interface, which makes it exceptionally convenient to use.
The Attune Xenith cytometer can be a high-parameter spectral analyzer, furnishing both spectral unmixing and standard flow abilities for in depth and multipurpose mobile Evaluation.
The Attune CytPix Flow Cytometer allows consumers to visualise samples throughout acquisition, validate the accuracy of gating methods, and aid benefits with trustworthy documentation for publication functions.
What seemed to be a homogeneous populace is definitely far more assorted—an Perception that should be thought of when interpreting effects.